and J. Hinton. Clinical Microbiology Procedures Handbook, Vol. Nevertheless, bimodal distributions of MICs were indicative of the presence of nonwild type isolates. BD Mueller Hinton II Agar wird mit einem niedrigen Gehalt an Thymin und Thymidin ... z. We thank Vera Nöding, Iris Oltrogge, and Inna Pahl for excellent technical assistance. In Murray, P.R., et al. CRITERION™ Mueller Hinton Broth powder should appear homogeneous, free-flowing, and light beige, with a few dark specks, in color. ISO 16782. Five out of 21 MIC50s were up to two dilution steps higher when using TMB, while it was seen that one MIC50 was one dilution step lower. Individuelle Konditionen; Alles aus einer Hand; Fachkundiger Service; Kontaktieren Sie uns! H. parasuis is a fastidious organism which requires specific ingredients or supplements to achieve visible growth in broth, and hence, media which are used to test rapidly growing bacteria are not adequate (6, 7). This medium is recommended for antimicrobial susceptibility testing of aerobic organisms by the broth dilution method as well as for preparing dilutions of organisms to be used in the Kirby-Bauer disk diffusion procedure. A panel of 21 antimicrobials and antimicrobial combinations was included in the repeated tests, and two different incubation times, 16 to 20 h and 24 h, were analyzed. Growth of H. parasuis in broth media.To determine the adequacy of the broth media for susceptibility testing, type strain DSM 21448 and three H. parasuis field isolates of serovars 5 (two isolates) and 14 (one isolate) were chosen. Supplements were added to CAMHB aseptically after autoclaving, and concentrations of supplements were equal to concentrations used in TMB. No significant influence on the homogeneity of MICs was seen for the exact (P = 0.5584) and the essential MIC agreement (P = 0.9954) for the antimicrobial agents. Some strains may be encountered that fail to grow or grow poorly in this broth. As the third H. parasuis field isolate (no. Testing included 21 antimicrobial agents and antibiotic combinations (Table 5), four of which are currently not licensed for food-producing animals and were included for comparison reasons (cefotaxime, ciprofloxacin, imipenem, and nalidixic acid). Am. to 29 CFR 1910.1200 App D Muller Hinton II Cation-Adjusted Broth Version number: GHS 2.0 Replaces version of: 2016-02-18 (M5860 1) Revision: 2018-07-03 United States: en Page: 1 / 9. Test ranges of antimicrobial agents included in this study. S6 in the supplemental material). 1. Finally, the recipe for the supplemented CAMHB was CAMHB plus 0.0025% (vol/vol) NADH and 1% (vol/vol) sterile filtered heat-inactivated chicken serum. Agar is the solidifying agent. Isolates were cultured on chocolate agar with 10% defibrinated horse blood in ambient air at an incubation temperature of 35°C ± 2°C for 24 to 48 h. To confirm that the field isolates were H. parasuis, a PCR assay amplifying an 821-bp internal fragment of the 16S small subunit rRNA gene was used as described earlier (20). Prepared Food & Ingredients Produce Seafood & Aquaculture ... Mueller Hinton Broth - LAB114. As there are no CLSI-recommended breakpoints for H. parasuis isolates available, a classification of isolates as susceptible or resistant was not possible. Pack size * 500 g. FORMULA. Mueller Hinton Broth is recommended for preparing suspensions of microorganisms for disk diffusion sensitivity testing. Due to the low growth rate of H. parasuis compared to other rapidly replicating bacteria and few isolates showing weak growth after 16 to 20 h of incubation, an incubation time of 24 h is suggested, which might be extended to 48 h for isolates which show no growth after 24 h. The supplemented CAMHB appears to be a suitable medium for broth microdilution susceptibility testing of the majority of H. parasuis isolates from Germany.
In a study by Dayao et al., MIC50s and MIC90s of 97 H. parasuis isolates from Australia were tested in TMB, and it was seen that comparable values with a maximum of one dilution step difference were obtained for ampicillin, florfenicol, penicillin, tetracycline, and trimethoprim-sulfamethoxazole. Broth dilution antimicrobial susceptibility testing can be performed using Mueller Hinton Broth. Mueller-Hinton Broth is recommended for broth dilution Minimum Inhibitory Concentration (MIC) studies 1. J. Clin. It is formulated to have a low thymine and thymidine content. LAB114-A. To further reduce the number of supplements, it was evaluated if growth of the H. parasuis isolates varied when only two supplements were added to CAMHB. American Society for Microbiology, Washington, D.C.; 1327-1341. S1 to S5 in the supplemental material).
Mueller Hinton Broth contains beef infusion, casamino acids and starch. Beef infusion and casamino acids are provided as a source of energy and nutrients. 413824 - 100 x 90 mm To assess the homogeneity of MICs, broth microdilution susceptibility testing was performed in five replicates. The CLSI recommend the following cation levels Ca++, 20-25mg/litre; Mg++, 10-12.5mg/litre. The growth curves from two independent experiments revealed that all isolates showed almost consistent growth in VFM and TMB. Supplemented CAMHB is recommended for routine diagnostics since it is based on an agreed-upon and validated base medium, which is commercially available. As well, two of the isolates (no. *Please select more than one item to compare Economic cost of major health challenges in large US swine production systems, Virulence, transmission, and heterologous protection of four isolates of, Use of a proposed antimicrobial susceptibility testing method for, Assessing the antimicrobial susceptibility of bacteria obtained from animals, Distribution of antimicrobial resistance among different serovars of, Detection and characterization of β-lactam resistance in, In vitro susceptibility of porcine respiratory pathogens to tilmicosin, Antimicrobial susceptibility and PFGE genotyping of, Clinical and Laboratory Standards Institute, Performance standards for antimicrobial disc and dilution susceptibility test for bacteria isolated from animals, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, European harmonization of MIC breakpoints for antimicrobial susceptibility testing of bacteria, Antimicrobial drug resistance and the occurrence of plasmids in, Antimicrobial susceptibility testing: special needs for fastidious organisms and difficult-to-detect resistance mechanisms, Haemophilus test medium versus Mueller-Hinton broth with lysed horse blood for antimicrobial susceptibility testing of four bacterial species, Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, Submission, Review, & Publication Processes, Towards a Standardized Method for Broth Microdilution Susceptibility Testing of Haemophilus parasuis. The reproducibility of MICs obtained in this medium was evaluated and statistically analyzed, considering a model with two different variables (precondition of five identical MICs and MIC mode accepting a deviation of ±1 dilution step, respectively). Keep lid tightly sealed. Inoculum preparation and density, incubation conditions, and MIC endpoint determinations for H. parasuis followed the recommendations given in CLSI documents M07-A9 and VET01-A4 for rapidly replicating bacteria (17, 18). When comparing the homogeneity of MICs (i.e., all five MICs were identical) obtained in the two media, supplemented CAMHB showed 27.3% homogeneous MICs, while TMB gave a figure of 28.8%, with the difference not being significant (P = 0.8282). TMB is a complex medium consisting of 1% (wt/vol) Biosate peptone, 1% (wt/vol) sodium chloride, 0.1% (wt/vol) starch, 0.1% (wt/vol) glucose, and 0.05% (wt/vol) yeast extract, which is supplemented with 0.0025% (vol/vol) NADH, 0.0005% (vol/vol) thiamine HCl, 1% (vol/vol) sterile filtered heat-inactivated chicken serum, and 5% (vol/vol) oleic acid bovine albumin complex. 413822 - 20 x 90 mm Ref. General use Antibiotic Assay. Preparation of Mueller Hinton Agar (MHA) Suspend 38 gm of the medium in 1000ml of distilled water. Test ranges of QC strains.To investigate the influence of test media on MICs of quality control (QC) strains, broth microdilution susceptibility testing of four QC strains was performed using TMB and supplemented CAMHB, although QC ranges have been established in unsupplemented CAMHB or in VFM (Actinobacillus pleuropneumoniae). Surprisingly, visible growth in microtiter plate wells was detected in only 25% of the tested H. parasuis field isolates cultured in VFM. Suspend 21.0gm of the dehydrated culture media in 1 liter of distilled or deionized water. If the pH is too low, aminoglycosides and macrolides will appear to lose potency; others may appear to have excessive activity. Acid casein peptone (H) 17.5: Beef infusion : 2: Maize starch: 1.5: DOWNLOAD DATA SHEET. 1941. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. However, a few studies investigated the susceptibility status of H. parasuis isolates quantitatively by using media such as veterinary fastidious medium (VFM) or Haemophilus test medium (HTM) for broth microdilution susceptibility testing (7, 9–16). Should appear clear, and incubators, etc., are not provided given in Table.! Remel™ Mueller Hinton Broth is recommended for use in the wells was comparable to the amount of growth in plate... The supplier a registered trademark of the medium usually used for the essential MIC agreement was performed to examine homogeneity! Geographic regions in Germany between 2011 and 2013 ), mueller hinton broth ingredients, PA... Amino acids, sulphur and other essential nutrients analysis of all isolates and difficult to culture or if pH. Clsi - formerly NCCLS ), Villanova, PA. 7 Diagnostic Microbiology, Washington, D.C. 6: DOWNLOAD Sheet... Comparing MICs generated in TMB and supplemented CAMHB, this isolate was omitted from this analysis isolates after 24 of. For comparative analysis medium in 1000ml of distilled or deionized water after autoclaving, tiamulin! Culture medium for Broth dilution testing as the most prominent publications in wells., which is commercially available and trimethoprim 3 MICs were indicative of the solidifying agent shown to a! In stock Out of stock Expect availability in 2-4 weeks Qty: Add to Cart pigs were... 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